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Objective:To explore the relationship between drug evidence and core prescription for depression.Methods:We retrieved literature of TCM for depression from CNKI, VIP and Wangfang databases to November 2019, 30th as well as there cords from Ancient and Modern Medical Records Cloud Platform (V 1.5). The Excel 2010 was used to establish the standardized database of medical records. After the standardization of medicines, Ancient and Modern Medical Records Cloud Platform (V1.5) statistics methodswere used for association rules analysis, complex networks, and analysis of drugs’ frequency, medical characteristics, core prescription drugs.Results:A total of 632 effective prescriptions were included, involving a total of 527 drugs. The results of frequency of herbs showed that 23 kinds of high-frequency herbs were obtained. Bupleuri Radix was the most frequently used medicine. Most herbs are warm or flat, with pungent, sweet and bitter in taste, belonging to the lung, liver, heart and spleen meridians. A total of 25 drug-pair association and 13 TCM association were obtained by association rule analysis. Conclusions:TCM treatment for depression is mainly based on soothing the liver and regulating qi, clearing the heart and calming the nerves. Bupleuri Radix, Curcumae Radix, Paeoniae Radix Alba, Chuanxiong Rhizoma, Ziziphi Spinosae Semen are the basic prescriptions. Angelicae Sinensis Radix, Ophiopogonis Radix, Albiziae Cortex, Polygalae Radix, Poria are used as reference.
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Objective To explore the application value of platelet parameters detection in early diagnosis of breast cancer.Methods Platelet parameters of intravenous blood from 103 breast cancer patients and 117 fibroadenoma patients within 24 hours of admission between January 2015 and December 2015 were collected and retrospectively analyzed,including platelet count(PLT),plateletcrit(PCT),mean platelet volume(MPV),mean platelet component concentration(MPC),mean platelet component mass (MPM),platelet distribution width(PDW),and compared with control group of 100 healthy women.The diagnostic efficiency of platelet parameters were assessed using receiver operating characteristic(ROC) curve.Results PLT and PCT in breast cancer group and fibroadenoma group were significantly higher than those in control group(P＜0.01);MPC,PMM and PDW were significantly lower than those in control group(P＜0.01);MPV had no significant statistically difference(P＞0.05).The levels of MPC in breast cancer group were obviously lower than that in fibroadenoma group(P＜0.01).The diagnostic efficiency of MPC in breast cancer had the highest area under curve(AUC=0.835).While the cutoff value of MPC was 249.5 g/L,sensitivity and specificity were 82.5 ％ and 73.6 ％,respectively.Conclusion The detection of platelet parameters,especially MPC,have certain application value in early diagnosis of breast cancer.
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Objective To evaluate combined effect on different population through 2 459 data of SAA,hs-CRP and PCT from 8 three-level hospitals in Guangdong region.Methods Subjects were divided into five groups by ages,and every group had bacterial and virus type.In order to confirm diagnostic effect on infection,methods were performed including in tendency of SAA and hs-CRP,Paired t test between bacterial and virus group,efficiency of 3 indexes in judging infection depending on ROC and parameters,multiple logistic regression,consistency between positive bacterial infection and bacterial culture.Re-sults There were statistically significant differences in SAA and hs-CRP between bacterial and virus in infants and children (P <0.001).SAA had the biggest AUC area 0.824 with sensibility 71.8% and specificity 82.6% in younger group.Corre-sponding,hs-CRP had the biggest area 0.806 with sensibility 84%.There was the accuracy of 78.8% for differential diagno-sis in younger group,while 65.1% in elder group.AUC of SAA was 0.883 for positive bacterial culture with sensibility 71.2% and specificity 90.7%,accuracy of 95.2% for differential diagnosis.Conclusion There was obvious trend of age in SAA and hs-CRP,3 indexes could be used for differential diagnosis alone or combined,especially in younger group.SAA is the best index as a separated index.There is less value at ratio of SAA and hs-CRP.
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The clinical prescription application of pinellia was investigated in a hospital, and the overdose phenomenon and underlying reasons and objectives were preliminarily analyzed. The resources current situation and industry characteristics of pinellia were also overviewed. A series of strategies for the resources problems of pinellia were provided in the paper as well.
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Objective The purpose of this study was to assess the analysis capabilities of the dot immunogold filtration method on detecting serum amyloid A ( SAA ) protein in blood.It also aimed to research the clinical value of SAA in diagnosing the infectious diseases of children .Methods ( 1 ) The performance evaluation including the accuracy , within-run precision, inter assay variations , the linear and the distraction-analysis of SAA-SPOT was estimated following the EP file; From March to July in 2014, children from five 3A Grade hospitals in Guangdong Province were enrolled into this observational study.Data including white blood WBC count , CRP and SAA were obtained.(2) From March to July in 2014, children from five 3A Grade hospitals in Guangdong Province were enrolled randomly into this observational study.This study used a cross-sectional survey research method , and 386 children with bacterial infection and 219 children with virus infection were as the research object.The general , clinical diagnosis , treatment information as well as the data of blood SAA , C-reactive protein ( CRP ) and white blood cell ( WBC ) of children were collected.Data were analyzed by variance , independent t test, ROC curve analysis and stepwise regression statistics method.Results ( 1 ) The average recovery rate is 103.74 %.Coefficient of variation (CV) for 10 mg/L,100 mg/L within-run assays were 8.77%, 3.61% and between-run assays were 9.01%, 3.74%;the inter-day CV were 9.07%, 4.03%respectively;the linear range was 5 mg/L-200 mg/L, hemoglobin(5 g/L),serum bilirubin(800 μmol/L),triglyceride(TG, 22 mmol/L), and had no interference in SAA detection.When compared to the BNPRO quantitate system of SIEMENS , the coefficient of association of detection of SAA by SAA-SPOT was R2 =0.96.( 2 ) Compared with control group , the serum SAA of infection group ( bacterial infection group , t =13.05, P=0.001;virus infection group t =7.68, P=0.001) and SAA/CRP ratio (bacterial infection group t=2.29, P=0.023;virus infection group t=3.32, P=0.01) were significantly increased.(3) The serum CRP and SAA rose similarly in bacterial infection, while in viral infection, only SAA increased significantly , CRP had no apparent change.In combination with CRP and WBC , SAA had the better diagnostic efficiency apparently.Conclusions As a POCT detection project , analysis capabilities of the SAA assayed by domestic SAA-SPOT can meet the requirements of clinical test.Combined with CRP , WBC and SAA can improve the efficiency in the diagnosing of infectious disease especially in the virus infection.As a new biomarker of infections , SAA is useful for the early auxiliary diagnosis and differential diagnosis of childhood infection.
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<p><b>OBJECTIVE</b>To determine the reversal effects of ramification of curcumin hydrolyzed on multidrug-resistant cell line K562/A02, and explore its reversal mechanism.</p><p><b>METHOD</b>After treatment with ramification of curcumin hydrolyzed, the sensitivity of K562/A02 cells to usuall chemotherapeutic drugs were determined by MTT. The expression of P-gp in K562/A02 and K562 cells was detected by immunohistochemical method. Intracellular mean fluorescence intensity (MFI) of DNR in K562 and K562/A02 cells was detected by Flow Cytometry.</p><p><b>RESULT</b>After treatment with the ramification of curcumin, hydrolyzed (2.5 mg x L(-1)) IC50, of usuall chemotherapeutic drugs to K562/A02 decreased. The sensitivity of K562/A02 cells increased. The expression of the P-gp in K562/A02 cells decreased (P < 0.05); MFI of the DNR in K562/A02 cells more significantly increased (P < 0.05). The expression of mdrl-mRNA decreased.</p><p><b>CONCLUSION</b>The ramification of curcumin hydrolyzed have effects on the reversal multidrug-resistant of K562/A02 cells in vitro. It could enhance the sensitivity of K562/A02 cells to chemotherapeutic drugs and the mechanism might be associated with inhibiting P-gp-mediated drug efflux and increasing of intracellular concentration of chemotherapeutic drugs.</p>
Subject(s)
Humans , ATP Binding Cassette Transporter, Subfamily B, Member 1 , Genetics , Metabolism , Curcumin , Chemistry , Pharmacology , Drug Resistance, Multiple , Drug Resistance, Neoplasm , Drugs, Chinese Herbal , Chemistry , Pharmacology , Gene Expression , K562 Cells , Neoplasms , Drug Therapy , Genetics , MetabolismABSTRACT
We detect the effect of soil drought stress on part physiological activities of Masson pine of different family.The dark respiration rate of Masson pine will decrease with different range of the decrease of leaf water potential.The photosynthesis rate will increase with the descendant leaf potential and soil potential,which demonstrates that Masson pine has much higher productivity of drought stress.Meanwhile,the relative plasma membrane permeability plasma will be enlarged and the membrane will be hurt more deeply when Masson pine meets with higher drought stress.The relationship between relative plasma membrane permeability and leaf potential shows significant linear correlation.
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Objective To evaluate the effect of three anesthetic methods on induced abortion. Methods three hundred women were randomly divided into three groups, groupⅠ(n=100), intravenous injection propofol(1.0~1.5mg/kg),groupⅡ(n=100),intravenous injection propofol (1.0~1.5mg/kg)combined with fentanyl ( 1?g/kg),group Ⅲ(n=100),2%lidocaine(5ml) into cavity of uterus as control group. Analgesia efficacy was assessed by visual analog scales (VAS) and sedation scales,SBP,DBP,SpO_2 at all in three groups.Results Analgesia efficacy in group Ⅱ was obviously higher than that in group Ⅰ(P